Different Methods of Detaching Adherent Cells and Their Effects on the Cell Surface Expression of Fas Receptor and Fas Ligand

Researchers demonstrated that the use of accutase for cellular detachment may have compromised some surface proteins. Compared with ethylenediaminetetraacetic acid (EDTA)-based nonenzymatic cell dissociation buffers, accutase was associated with significant decreases in the surface Fas ligands and Fas receptors.