[Gut] Scientists aimed to identify and characterize a nuclear Wnt negative-feedback loop that remains functional in both Adenomatous polyposis coli (APC)-wild-type and APC-mutant CRC, restrains Wnt/β-catenin hyperactivation downstream of APC, and provides a strategy for overcoming the limitations of existing Wnt negative-feedback mechanisms in APC-mutant CRC.

[Cell Host & Microbe] The authors found that Gemella morbillorum is enriched in feces and tumor tissues from patients with CRC and revealed a multi-step host-pathogen mechanism that promotes colorectal tumorigenesis.

[Cell Reports] Investigators identified EpCAM as a regulator of membrane-cytoskeletal mechanics using membrane proteomics, gastric cancer cell models, biophysical measurements, and in vivo metastasis assays.

[JCI Insight] Scientists showed that serotonin is detected in both the small intestine and large intestine as early as 14 to 15 weeks gestational age, generating a spatiotemporal atlas of serotonin and serotonin-related genes in the human gut from first trimester to late adulthood using single-cell transcriptomics with histological validation.

[Food & Function] Researchers investigated the effects of 1,2,3,4,6-penta-O-galloyl-β-D-glucose on lipid metabolism and ferroptosis-related cell death in CRC cells and an MC38 syngeneic tumor model.

[Biochemical Pharmacology] The authors identified aripiprazole, an approved antipsychotic drug with emerging anticancer activity, as a disruptor of metabolic plasticity in CRC.

[Journal of Enzyme Inhibition and Medicinal Chemistry] Scientists identified a series of novel B-cell lymphoma 2 (Bcl-2)-targeted peptides through structure-based virtual screening and systematic biological validation. Bcl-2 is critical for tumour survival and serves as a promising anticancer drug target.

[Translational Oncology] Researchers integrated transcriptomics, machine learning, single-cell RNA sequencing, spatial transcriptomics and in vitro experiments to prioritize candidate CRC-associated markers and characterize their potential biological and clinical relevance.

[Toxicology and Applied Pharmacology] Previous studies have found that auriculasin isolated from Flemingia philippinensis has a significant anticancer effect in CRC. Scientists aimed to study the mechanism of auriculasin in CRC.

[Gene] Scientists developed a single-guide RNA (gRNA) CRISPR/eCas9 strategy to simultaneously disrupt both BIRC2 and BIRC3 genes in human CRC HCT-116 cells, and compared the resulting cellular and transcriptomic effects with those of the individual knockouts.

[Molecular and Cellular Biochemistry] Scientists investigated whether piperine acts as a thermosensitizer in AGS gastric cancer cells and explored associations with oxidative stress, apoptosis, and the heat-shock response.

[Pharmacological Research] The authors comprehensively summarize the current molecular mechanisms by which endoplasmic reticulum stress and mitochondrial dysfunction aid IBD pathogenesis, emphasizing calcium homeostasis imbalance, oxidative stress, disrupted mitochondrial quality control, and autophagy–unfolded protein response integration.