western blot analysis

[Cancer Science] Scientists investigated the effect of PMD-026 on YB-1/AR signaling and its antitumor effect in prostate cancer in vitro and in vivo and demonstrated an excellent antitumor effect of the novel ribosomal S6 kinase inhibitor PMD-026 and the combination effect with the antiandrogen enzalutamide in CRPC.

[Cell Communication and Signaling] The biological role of copine 1 (CPNE1) and receptor for activated C kinase 1 (RACK1) in NSCLC was investigated using gene expression knockdown and overexpression, cell proliferation assays, clonogenic assays, and Transwell assays.

[Cardiovascular Drugs and Therapy] Oxidized low-density lipoprotein was used to treat human umbilical vein endothelial cells to construct a cell injury model. The expression level of circCHMP5, miR-532-5p, and Rho-associated protein kinase 2 was measured using quantitative real-time PCR.

[Prostate] Researchers found that the increase of transcription factor 6 α (ATF6α) expression in response to androgen deprivation generated prostate cancer cells with a CRPC-like phenotype.

[Experimental Hematology & Oncology] In vitro mammary spheroid formation, flow cytometry assay on CD24−/CD44+ sub-population, ALDH activity detection, cell viability assay and western blot analysis, and in vivo tumor-initiating analysis were performed to examine the effects of Tanshinone IIA on the stemness of breast cancer cells.

[Molecular Biology Reports] H. leucospilota was collected, the methanolic extract was prepared and in vitro cytotoxicity of H. leucospilota extract in the range of 12.5, 25, 50, 100, and 200 μg/mL concentrations for 48 hours on SK-BR-3 and MCR5 cells was determined.