in vivo

[Scientific Reports] Researchers successfully converted three in-house-derived primed human PSC lines to a naive state and an expanded pluripotent stem cell (EPS) state. Primed, naive and EPS cells displayed state-specific morphologies and expressed pluripotent markers.

[Clinical Cancer Research] In vivo activity of nanoliposomal irinotecan was validated in an orthotopic nude murine model expressing TAK1-specific shRNA. Differential expression profiling revealed the gene coding for interleukin-8 as the most significantly downregulated in shTAK1 pancreatic cancer cell lines.

[Communications Biology] Scientists used CRISPR–Cas9 technology to generate mice with mutations in the promoter regions of the insulin I and II genes.

[Communications Biology] An inert form of the human NKG2D extracellular domain (iNKG2D) was engineered as the ectodomain of the chimeric antigen receptor (CAR) to generate convertibleCARTM-T cells. These cells were specifically directed to kill antigen-expressing target cells only in the presence of an activating bispecific adapter comprised of an iNKG2D-exclusive ULBP2-based ligand fused to an antigen-targeting antibody

[Molecular Therapy] The biological capacity of lncRNA named lncRNA activated in metastatic PCa (lncAMPC) in prostate cancer (PCa) was demonstrated both in vitro and in vivo. The lncAMPC was overexpressed in tumor tissue and urine of metastatic PCa patients and promoted PCa tumorigenesis and metastasis.

[Cell Stem Cell] The authors developed human cytokine-inducible SH2-containing protein-knockout (CISH−/−) NK cells using an induced pluripotent stem cell-derived NK cell (iPSC-NK cell) platform. CISH−/− iPSC-NK cells demonstrated increased IL-15-mediated JAK-STAT signaling activity.