in vivo

[British Journal of Cancer] From head-and-neck squamous cell carcinoma cell lines not expressing CYP1B1, investigators generated isogenic derivatives expressing the two forms. Proliferation, invasiveness, stem cell characteristics, sensitivity to anticancer agents and transcriptome were analyzed.

[Cancer Discovery] Scientists showed that while WNT suppression blocked tumor growth in most organoid and in vivo colorectal cancer (CRC) models, the accumulation of CRC-associated genetic alterations enabled drug resistance and WNT-independent growth.

[Nature Communications] The authors isolated a mouse strain with a single missense mutation, MlklD139V, that altered the two-helix ‘brace’ that connects the killer four-helix bundle and regulatory pseudokinase domains.

[Cell Reports] Researchers combined lineage tracing in a spontaneous metastatic mouse model of melanoma, advanced imaging, and single-cell RNA sequencing approaches to search for pathophysiologically relevant melanoma cellular states.

[Signal Transduction and Targeted Therapy] Scientists demonstrated that PTEN was a direct target of miR-410 by using bioinformatic tools and dual-luciferase reporter assays, and the miR-410-induced epithelial–mesenchymal transition and radioresistance were reversed by PI3K, Akt, and mTOR inhibitors or by restoring the expression of PTEN in non-small cell lung cancer cells.

[British Journal of Cancer] The authors demonstrated that miRNA-17-5p regulated vimentin expression, thereby regulating metastasis of colorectal cancer.