hepatocytes

[Biomaterials] Tthe culture and expansion of human cholangiocyte organoids in liver extracellular matrix-derived hydrogels is described. These hydrogels supported proliferation of cholangiocyte organoids and maintain the cholangiocyte-like phenotype.

[Cell Reports] To obtain a better appreciation of the molecular processes during the differentiation of iPSCs into hepatocytes, researchers employed a quantitative proteomic approach to follow the expression of 9,000 proteins, 12,000 phosphorylation sites, and 800 acetylation sites over time.

[Cell Death & Disease] Employing a combination of chimeric lineage tracing, immunofluorescence, and immunohistochemistry, the authors demonstrated that Sox9+HNF4α+ hepatocytes, generated by transition from mature hepatocytes, played an important role in the initial phase after partial hepatectomy.

[NPJ Regenerative Medicine] The authors showed that in response to extensive hepatocyte damages, expression of dnmt1 was upregulated in biliary epithelial cells (BECs) to methylate DNA at the p53 locus, which repressed p53 transcription, and in turn, derepressed mTORC1 signaling to activate BEC dedifferentiation.

[Hepatology] Scientists evaluated the function and efficacy of autologous reprogrammed, ATP7B gene-restored hepatocytes using a murine model of Wilson's disease.

[Cell Death & Disease] The inhibitory effect of Selenoprotein S (SelS) on hepatic steatosis and insulin resistance was confirmed by SelS overexpression in primary hepatocytes in vitro.