gene knockdown

[Stem Cells and Development] Through a siRNA-based high throughput screen that identifies adipogenic regulators whose expression knockdown leads to enhanced adipogenic differentiation of human mesenchymal stem cells, two new regulators, SUV39H1, a histone methyltransferase that catalyzes H3K9Me3, and CITED2, a CBP/p300 interacting transactivator with Glu/Asp rich carboxy-terminal domain 2 were uncovered.

[Stem Cell Research & Therapy] Investigators designed and performed this study to determine whether exosomes attenuate the lesion size of spinal cord injury by ameliorating neuronal injury induced by a secondary inflammatory storm and promoting neurite outgrowth.

[Endocrinology and Metabolism] Treatment with clusterin remarkably upregulated microtubule-associated protein 1-light chain 3 (LC3)-II conversion in a dose and time-dependent manner with a significant increase in the autophagy-related 3 gene expression level, which is a mediator of LC3-II conversion.

[Scientific Reports] Cholestatic liver fibrosis rats transplanted into rat bone marrow-derived mesenchymal stem cells with knocked down Numb gene were treated with Huang Qi Decoction.

[Scientific Reports] Cholestatic liver fibrosis rats transplanted into rat bone marrow-derived MSCs with knocked down Numb gene were treated with Huang Qi Decoction (HQD). Simultaneously, Numb gene knockdown was also performed in the WB-F344 cell line and then treated with refined HQD .

[Archives of Toxicology] The authors concluded that MRP1 protected placental cells from methyl mercury-induced oxidative stress by exporting the toxic metal and by maintaining the placental cells' glutathione status in equilibrium.