cell culture

[Frontiers in Molecular Neuroscience] Investigators tested the hypothesis that ZIKV-associated degeneration of neuronal processes was dependent on activation of Sterile alpha and armadillo motif-containing protein 1 (SARM1), an NADase that acted as a central executioner in a conserved axon degeneration pathway.

[Journal of Controlled Release] Researchers developed a formulation for pulmonary administration of siRNA to suppress SARS-CoV-2 replication in lung epithelial cells.

[Scientific Reports] Researchers demonstrated that the use of accutase for cellular detachment may have compromised some surface proteins. Compared with ethylenediaminetetraacetic acid (EDTA)-based nonenzymatic cell dissociation buffers, accutase was associated with significant decreases in the surface Fas ligands and Fas receptors.

[Translational Oncology] Scientists adapted a previously established brain tissue-model to fresh patient tumor cells with the goal of establishing 3D in vitro culture conditions for each tumor type.

[Frontiers in Pharmacology] Enrichment of cancer stem cells was observed in neurospheres by FACS using CD133 antibody and RT-PCR.

[Cancer Immunology Immunotherapy] In an in vitro study using lung cancer cell lines and human monocyte-derived macrophages, the conditioned medium from cancer cells was found to up-regulate programmed cell death-1 ligand 1 expression on macrophages via STAT3 activation.