Human Umbilical Cord Mesenchymal Stem Cells-Derived Extracellular Vesicles Facilitate the Repair of Spinal Cord Injury via the miR-29b-3p/PTEN/Akt/mTOR Axis

By
Justin.choi
-
0
129
Investigators explored the repair effect of human umbilical cord MSCs-derived extracellular vesicles on spinal cord injury.
[Cell Death Discovery]
6445218 {6445218:M6E82YM5} 1 apa 50 default 1 165651 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3Afalse%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22M6E82YM5%22%2C%22library%22%3A%7B%22id%22%3A6445218%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Xiao%20et%20al.%22%2C%22parsedDate%22%3A%222021-08-11%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BXiao%2C%20X.%2C%20Li%2C%20W.%2C%20Rong%2C%20D.%2C%20Xu%2C%20Z.%2C%20Zhang%2C%20Z.%2C%20Ye%2C%20H.%2C%20Xie%2C%20L.%2C%20Wu%2C%20Y.%2C%20Zhang%2C%20Y.%2C%20%26amp%3B%20Wang%2C%20X.%20%282021%29.%20Human%20umbilical%20cord%20mesenchymal%20stem%20cells-derived%20extracellular%20vesicles%20facilitate%20the%20repair%20of%20spinal%20cord%20injury%20via%20the%20miR-29b-3p%5C%2FPTEN%5C%2FAkt%5C%2FmTOR%20axis.%20%26lt%3Bi%26gt%3BCell%20Death%20Discovery%26lt%3B%5C%2Fi%26gt%3B%2C%20%26lt%3Bi%26gt%3B7%26lt%3B%5C%2Fi%26gt%3B%281%29%2C%201%26%23x2013%3B10.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41420-021-00572-3%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41420-021-00572-3%26lt%3B%5C%2Fa%26gt%3B%20%26lt%3Ba%20title%3D%26%23039%3BCite%20in%20RIS%20Format%26%23039%3B%20class%3D%26%23039%3Bzp-CiteRIS%26%23039%3B%20data-zp-cite%3D%26%23039%3Bapi_user_id%3D6445218%26amp%3Bitem_key%3DM6E82YM5%26%23039%3B%20href%3D%26%23039%3Bjavascript%3Avoid%280%29%3B%26%23039%3B%26gt%3BCite%26lt%3B%5C%2Fa%26gt%3B%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Human%20umbilical%20cord%20mesenchymal%20stem%20cells-derived%20extracellular%20vesicles%20facilitate%20the%20repair%20of%20spinal%20cord%20injury%20via%20the%20miR-29b-3p%5C%2FPTEN%5C%2FAkt%5C%2FmTOR%20axis%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xiao%22%2C%22lastName%22%3A%22Xiao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Weiwei%22%2C%22lastName%22%3A%22Li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dingchao%22%2C%22lastName%22%3A%22Rong%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zhenchao%22%2C%22lastName%22%3A%22Xu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zhen%22%2C%22lastName%22%3A%22Zhang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hongru%22%2C%22lastName%22%3A%22Ye%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Liqiong%22%2C%22lastName%22%3A%22Xie%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yunqi%22%2C%22lastName%22%3A%22Wu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yilu%22%2C%22lastName%22%3A%22Zhang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xiyang%22%2C%22lastName%22%3A%22Wang%22%7D%5D%2C%22abstractNote%22%3A%22Spinal%20cord%20injury%20%28SCI%29%20is%20a%20salient%20traumatic%20disease%20that%20often%20leads%20to%20permanent%20disability%2C%20and%20motor%20and%20sensory%20impairments.%20Human%20umbilical%20cord%20mesenchymal%20stem%20cells%20%28HucMSCs%29%20have%20a%20wide%20application%20prospect%20in%20the%20treatment%20of%20SCI.%20This%20study%20explored%20the%20repair%20effect%20of%20HucMSCs-derived%20extracellular%20vesicles%20%28HucMSCs-EVs%29%20on%20SCI.%20HucMSCs%20and%20HucMSCs-EVs%20were%20cultured%20and%20identified.%20The%20rat%20model%20of%20SCI%20was%20established%2C%20and%20SCI%20rats%20were%20treated%20with%20HucMSCs-EVs.%20The%20motor%20function%20of%20SCI%20rats%20and%20morphology%20of%20spinal%20cord%20tissues%20were%20evaluated.%20Levels%20of%20NeuN%2C%20GFAP%2C%20and%20NF200%20in%20spinal%20cord%20tissues%20were%20detected%20and%20cell%20apoptosis%20was%20measured.%20SCI%20rats%20were%20treated%20with%20EVs%20extracted%20from%20miR-29b-3p%20inhibitor-transfected%20HucMSCs.%20The%20downstream%20gene%20and%20pathway%20of%20miR-29b-3p%20were%20examined.%20HucMSCs-EVs-treated%20rats%20showed%20obvious%20motor%20function%20recovery%20and%20reduced%20necrosis%2C%20nuclear%20pyknosis%2C%20and%20cavity.%20HucMSCs-EVs%20alleviated%20spinal%20cord%20neuronal%20injury.%20miR-29b-3p%20was%20poorly%20expressed%20in%20SCI%20tissues%2C%20but%20highly%20expressed%20in%20EVs%20and%20SCI%20rats%20treated%20with%20EVs.%20miR-29b-3p%20targeted%20PTEN.%20Inhibition%20of%20miR-29b-3p%20or%20overexpression%20of%20PTEN%20reversed%20the%20repair%20effect%20of%20EVs%20on%20SCI.%20EVs%20activated%20the%20AKT%5C%2FmTOR%20pathway%20via%20the%20miR-29b-3p%5C%2FPTEN.%20In%20conclusion%2C%20HucMSCs-EVs%20reduced%20pathological%20changes%2C%20improved%20motor%20function%2C%20and%20promoted%20nerve%20function%20repair%20in%20SCI%20rats%20via%20the%20miR-29b-3p%5C%2FPTEN%5C%2FAkt%5C%2FmTOR%20axis.%22%2C%22date%22%3A%222021-08-11%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41420-021-00572-3%22%2C%22ISSN%22%3A%222058-7716%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41420-021-00572-3%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-08-12T23%3A49%3A06Z%22%7D%7D%5D%7D
Xiao, X., Li, W., Rong, D., Xu, Z., Zhang, Z., Ye, H., Xie, L., Wu, Y., Zhang, Y., & Wang, X. (2021). Human umbilical cord mesenchymal stem cells-derived extracellular vesicles facilitate the repair of spinal cord injury via the miR-29b-3p/PTEN/Akt/mTOR axis. Cell Death Discovery, 7(1), 1–10. https://doi.org/10.1038/s41420-021-00572-3 Cite
Full Article