Novel Integrated Workflow Allows Production and In-Depth Quality Assessment of Multifactorial Reprogrammed Skeletal Muscle Cells from Human Stem Cells

Researchers developed a multifactor-based protocol that combined adenovector-mediated MYOD expression, small molecule inhibitor and growth factor treatment, and electrical pulse stimulation to efficiently reprogram different types of human-derived multipotent stem cells into physiologically functional skeletal muscle cells.