convertibleCARs: A Chimeric Antigen Receptor System for Flexible Control of Activity and Antigen Targeting

An inert form of the human NKG2D extracellular domain (iNKG2D) was engineered as the ectodomain of the chimeric antigen receptor (CAR) to generate convertibleCAR^TM-T cells. These cells were specifically directed to kill antigen-expressing target cells only in the presence of an activating bispecific adapter comprised of an iNKG2D-exclusive ULBP2-based ligand fused to an antigen-targeting antibody