Noshin Noorjahan

[eLife] The authors induced inactivating deletions of CEP83 in hiPSCs and applied a 7 day in vitro protocol of intermediate mesoderm kidney progenitor differentiation, based on timed application of WNT and FGF agonists.

[Cellular and Molecular Life Sciences] Investigators demonstrated that yes-associated protein functioned in an hESC-specific manner to maintain self-renewal and survival in hESCs. hESCs were highly sensitive to YAP downregulation to promote cell survival.

[Stem Cell Research] Researchers generated an iPSC line of a severely affected Loeys-Dietz Syndrome patient carrying a pathogenic SMAD3 p.Arg287Gln variant. Peripheral blood mononuclear cells were reprogrammed using non-integrating Sendai viral vectors.

[iScience] Using high-throughput sequencing, chromatin immunoprecipitation and luciferase reporter assays, scientists showed that Prdm14 directly bound to the promoter of Socs3 and repressed its transcription to increase the phosphorylation level of Stat3 protein.

[Experimental Dermatology] Investigators monoclonally isolated CD8+ T cells specifically reactive with NY-ESO-1 from the melanoma patient’s monocytes after stimulated with NY-ESO-1 peptide by manual procedure, and cultured NY-ESO-1-specific TCs until proliferated and formed colonies.

[Trends in Cell Biology] The authors review recent literature on the mechanistic role of different types of autophagy in embryonic development, ESCs, and iPSCs, focusing on their remodeling functions on protein, metabolism, and epigenetics.