Insights for Disease Modeling from Single-Cell Transcriptomics of iPSC-Derived Ngn2-Induced Neurons and Astrocytes across Differentiation Time and Co-Culture

Scientists examined the homogeneity and similarity of human-induced pluripotent stem cells (hiPSC) derived neuron (hiPSC-N) and hiPSC derived astrocyte A (hiPSC-A) to their in vivo counterparts, the impact of different lengths of time post Ngn2 induction on hiPSC-N (15 or 21 days), and the impact of hiPSC-N/hiPSC-A co-culture.