Shedding of Brucella melitensis Happens through Milk Macrophages in the Murine Model of Infection

Pregnant Balb/cByJ mice were intraperitoneally infected with 10⁵ CFU of the 16 M reference strain, a 16 M mCherry mutant or a human isolate. Milk was collected over the course of lactation, and subjected to culture and immunofluorescence assays.