A Clinically Effective Model Based on Cell-Free DNA Methylation and Low-Dose CT for Risk Stratification of Pulmonary Nodules

The authors performed genome-wide methylation sequencing on malignant and non-malignant lung tissues and designed a panel of 263 differential DNA methylation regions, which was used for targeted methylation sequencing on blood cell-free DNA in two prospectively collected and retrospectively analyzed multicenter cohorts.