ESC & iPSC News
ESC & iPSC News is an online resource dedicated to the latest research in the fields of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).
DDX18 Coordinates Nucleolus Phase Separation and Nuclear Organization to Control the Pluripotency of Human Embryonic Stem Cells
[Nature Communications] Researchers showed that the nucleolus-specific RNA helicase DDX18 was essential for maintaining the pluripotency of human embryonic stem cells. They revealed how nucleolar phase separation dynamics govern chromatin organization and cell fate.
Interplay of Chromatin Remodeling BAF Complexes in Mouse Embryonic and Epiblast Stem Cell Conversion and Maintenance
[Journal of Biological Chemistry] Scientists demonstrated that the LIF/STAT3 and Wnt signaling pathways, in conjunction with canonical BAF (cBAF) and PRC2 complexes, inhibited epiblast stem cells (EpiSCs) gene expression, thereby preventing ESCs from converting to EpiSCs.
Reconstitution of Pluripotency from Mouse Fibroblast through Sall4 Overexpression
[Nature Communications] Investigators reported that SALL4 alone, under an optimized reprogramming medium iCD4, was capable of reprogramming mouse fibroblasts into iPSCs. Mechanistically, SALL4 facilitated reprogramming by inhibiting somatic genes and activating pluripotent genes, such as Esrrb and Tfap2c.
A NOTCH3 Pathogenic Variant Influences Osteogenesis and Can Be Targeted by Antisense Oligonucleotides in Induced Pluripotent Stem Cells
[PLOS One] To determine the consequences of Lateral Meningocele Syndrome pathogenic variants in human cells and whether they can be targeted by antisense oligonucleotides, induced pluripotent NCRM1 and NCRM5 stem cells harboring a NOTCH36692-93insC insertion were created.
Advances in Engineering Myeloid Cells for Cell Therapy Applications
[ACS Synthetic Biology] Recent advancements in stem cell research have made it possible to differentiate human ESCs and induce pluripotent stem cells into various cell types, including myeloid cells, offering a promising new approach to generate myeloid cells for cell therapy.
Long-Term Correction of Hemophilia A via Integration of a Functionally Enhanced FVIII Gene into the AAVS1 Locus by Nickase in Patient-Derived iPSCs
[Experimental & Molecular Medicine] Researchers employed CRISPR/Cas9 nickase-mediated knock-in to insert either the wild-type B domain-deleted (BDD)-FVIII gene or a functionally enhanced BDD-FVIII gene driven by a human elongation factor-1 alpha promoter into the adeno-associated virus site 1 locus of iPSCs derived from a patient with Hemophilia A.
A Rapid Chemical Reprogramming System to Generate Human Pluripotent Stem Cells
[Nature Chemical Biology] Investigators developed a fast reprogramming system capable of generating human pluripotent stem cells (hCiPS) cells in as few as 10 days. This accelerated method enabled efficient generation of hCiPS cells with a consistent 100% success rate across 15 different donors, increasing efficiency by over 20-fold within 16 days.
Abu Dhabi Stem Cells Center Successfully Develops Virus-Free, Clinical-Grade Induced Pluripotent Stem Cells for First Time in the Middle East Using Exclusive Protocol
[ADSCC] The Abu Dhabi Stem Cells Center (ADSCC) have successfully developed clinical-grade iPSCs that complied with the Good Manufacturing Practice protocols and were, as such, compliant with the strictest international standards for clinical applications.
Bioreactor Allows Automated Long-Term Culturing of Stem Cells
[ISC] Researchers from the Fraunhofer Translational Center for Regenerative Therapies at the Fraunhofer Institute for Silicate Research (ISC) have developed a bioreactor that can be used for automated long-term culturing of human iPSCs.
Pluripotent Stem-Cell-Derived Therapies in Clinical Trial: A 2025 Update
[Cell Stem Cell] The authors review the landscape of interventional human PSC trials worldwide, highlighting available data on clinical safety and efficacy.
Cardiac Fibroblast BAG3 Regulates TGFBR2 Signaling and Fibrosis in Dilated Cardiomyopathy
[Journal of Clinical Investigation] Scientists used an isogenic pair of Bcl2-associated athanogene 3 (BAG3)-knockout and wild-type human iPSCs (hiPSCs) to interrogate the role of BAG3 in hiPSC-derived cardiac fibroblasts.
Protogenin Facilitates Trunk-to-Tail HOX Code Transition via Modulating GDF11/SMAD2 Signaling in Mammalian Embryos
[Communications Biology] Using human iPSC-derived presomitic mesoderm-like cells, scientists demonstrated delayed posterior HOX gene expression upon protogenin (PRTG) knockout, which was rescued by growth differentiation factor 11 (GDF11) supplementation.
Since 2007, ESC & iPSC News has been providing updates on the top publications, reviews, and news in pluripotent stem cell research. Through our website, Twitter, and email newsletters, our editorial team is proud to help keep researchers and medical professionals up-to-date with the latest discoveries, jobs, and event postings in the pluripotent stem cell field.
